X-Git-Url: https://git.arvados.org/lightning.git/blobdiff_plain/d62d55adee66142b030b84436a0805d28c961342..HEAD:/import.go diff --git a/import.go b/import.go deleted file mode 100644 index 9c933da3de..0000000000 --- a/import.go +++ /dev/null @@ -1,394 +0,0 @@ -package main - -import ( - "bufio" - "compress/gzip" - "encoding/gob" - "errors" - "flag" - "fmt" - "io" - "net/http" - _ "net/http/pprof" - "os" - "os/exec" - "path/filepath" - "regexp" - "runtime" - "sort" - "strings" - "sync" - "sync/atomic" - "time" - - "git.arvados.org/arvados.git/sdk/go/arvados" - log "github.com/sirupsen/logrus" -) - -type importer struct { - tagLibraryFile string - refFile string - outputFile string - projectUUID string - runLocal bool - skipOOO bool - encoder *gob.Encoder -} - -func (cmd *importer) RunCommand(prog string, args []string, stdin io.Reader, stdout, stderr io.Writer) int { - var err error - defer func() { - if err != nil { - fmt.Fprintf(stderr, "%s\n", err) - } - }() - flags := flag.NewFlagSet("", flag.ContinueOnError) - flags.SetOutput(stderr) - flags.StringVar(&cmd.tagLibraryFile, "tag-library", "", "tag library fasta `file`") - flags.StringVar(&cmd.refFile, "ref", "", "reference fasta `file`") - flags.StringVar(&cmd.outputFile, "o", "-", "output `file`") - flags.StringVar(&cmd.projectUUID, "project", "", "project `UUID` for output data") - flags.BoolVar(&cmd.runLocal, "local", false, "run on local host (default: run in an arvados container)") - flags.BoolVar(&cmd.skipOOO, "skip-ooo", false, "skip out-of-order tags") - priority := flags.Int("priority", 500, "container request priority") - pprof := flags.String("pprof", "", "serve Go profile data at http://`[addr]:port`") - err = flags.Parse(args) - if err == flag.ErrHelp { - err = nil - return 0 - } else if err != nil { - return 2 - } else if cmd.tagLibraryFile == "" { - fmt.Fprintln(os.Stderr, "cannot import without -tag-library argument") - return 2 - } else if flags.NArg() == 0 { - flags.Usage() - return 2 - } - - if *pprof != "" { - go func() { - log.Println(http.ListenAndServe(*pprof, nil)) - }() - } - - if !cmd.runLocal { - runner := arvadosContainerRunner{ - Name: "lightning import", - Client: arvados.NewClientFromEnv(), - ProjectUUID: cmd.projectUUID, - RAM: 30000000000, - VCPUs: 16, - Priority: *priority, - } - err = runner.TranslatePaths(&cmd.tagLibraryFile, &cmd.refFile, &cmd.outputFile) - if err != nil { - return 1 - } - inputs := flags.Args() - for i := range inputs { - err = runner.TranslatePaths(&inputs[i]) - if err != nil { - return 1 - } - } - if cmd.outputFile == "-" { - cmd.outputFile = "/mnt/output/library.gob" - } else { - // Not yet implemented, but this should write - // the collection to an existing collection, - // possibly even an in-place update. - err = errors.New("cannot specify output file in container mode: not implemented") - return 1 - } - runner.Args = append([]string{"import", "-local=true", "-tag-library", cmd.tagLibraryFile, "-ref", cmd.refFile, "-o", cmd.outputFile}, inputs...) - var output string - output, err = runner.Run() - if err != nil { - return 1 - } - fmt.Fprintln(stdout, output+"/library.gob") - return 0 - } - - infiles, err := listInputFiles(flags.Args()) - if err != nil { - return 1 - } - - tilelib, err := cmd.loadTileLibrary() - if err != nil { - return 1 - } - go func() { - for range time.Tick(10 * time.Minute) { - log.Printf("tilelib.Len() == %d", tilelib.Len()) - } - }() - - var output io.WriteCloser - if cmd.outputFile == "-" { - output = nopCloser{stdout} - } else { - output, err = os.OpenFile(cmd.outputFile, os.O_CREATE|os.O_WRONLY, 0777) - if err != nil { - return 1 - } - defer output.Close() - } - bufw := bufio.NewWriter(output) - cmd.encoder = gob.NewEncoder(bufw) - - err = cmd.tileInputs(tilelib, infiles) - if err != nil { - return 1 - } - err = bufw.Flush() - if err != nil { - return 1 - } - err = output.Close() - if err != nil { - return 1 - } - return 0 -} - -func (cmd *importer) tileFasta(tilelib *tileLibrary, infile string) (tileSeq, error) { - var input io.ReadCloser - input, err := os.Open(infile) - if err != nil { - return nil, err - } - defer input.Close() - if strings.HasSuffix(infile, ".gz") { - input, err = gzip.NewReader(input) - if err != nil { - return nil, err - } - defer input.Close() - } - return tilelib.TileFasta(infile, input) -} - -func (cmd *importer) loadTileLibrary() (*tileLibrary, error) { - log.Printf("tag library %s load starting", cmd.tagLibraryFile) - f, err := os.Open(cmd.tagLibraryFile) - if err != nil { - return nil, err - } - defer f.Close() - var rdr io.ReadCloser = f - if strings.HasSuffix(cmd.tagLibraryFile, ".gz") { - rdr, err = gzip.NewReader(f) - if err != nil { - return nil, fmt.Errorf("%s: gzip: %s", cmd.tagLibraryFile, err) - } - defer rdr.Close() - } - var taglib tagLibrary - err = taglib.Load(rdr) - if err != nil { - return nil, err - } - if taglib.Len() < 1 { - return nil, fmt.Errorf("cannot tile: tag library is empty") - } - log.Printf("tag library %s load done", cmd.tagLibraryFile) - return &tileLibrary{taglib: &taglib, skipOOO: cmd.skipOOO}, nil -} - -func listInputFiles(paths []string) (files []string, err error) { - for _, path := range paths { - if fi, err := os.Stat(path); err != nil { - return nil, fmt.Errorf("%s: stat failed: %s", path, err) - } else if !fi.IsDir() { - if !strings.HasSuffix(path, ".2.fasta") || strings.HasSuffix(path, ".2.fasta.gz") { - files = append(files, path) - } - continue - } - d, err := os.Open(path) - if err != nil { - return nil, fmt.Errorf("%s: open failed: %s", path, err) - } - defer d.Close() - names, err := d.Readdirnames(0) - if err != nil { - return nil, fmt.Errorf("%s: readdir failed: %s", path, err) - } - sort.Strings(names) - for _, name := range names { - if strings.HasSuffix(name, ".vcf") || strings.HasSuffix(name, ".vcf.gz") { - files = append(files, filepath.Join(path, name)) - } else if strings.HasSuffix(name, ".1.fasta") || strings.HasSuffix(name, ".1.fasta.gz") { - files = append(files, filepath.Join(path, name)) - } - } - d.Close() - } - for _, file := range files { - if strings.HasSuffix(file, ".1.fasta") || strings.HasSuffix(file, ".1.fasta.gz") { - continue - } else if _, err := os.Stat(file + ".csi"); err == nil { - continue - } else if _, err = os.Stat(file + ".tbi"); err == nil { - continue - } else { - return nil, fmt.Errorf("%s: cannot read without .tbi or .csi index file", file) - } - } - return -} - -func (cmd *importer) tileInputs(tilelib *tileLibrary, infiles []string) error { - starttime := time.Now() - errs := make(chan error, 1) - todo := make(chan func() error, len(infiles)*2) - var encodeJobs sync.WaitGroup - for _, infile := range infiles { - infile := infile - var phases sync.WaitGroup - phases.Add(2) - variants := make([][]tileVariantID, 2) - if strings.HasSuffix(infile, ".1.fasta") || strings.HasSuffix(infile, ".1.fasta.gz") { - todo <- func() error { - defer phases.Done() - log.Printf("%s starting", infile) - defer log.Printf("%s done", infile) - tseqs, err := cmd.tileFasta(tilelib, infile) - variants[0] = tseqs.Variants() - return err - } - infile2 := regexp.MustCompile(`\.1\.fasta(\.gz)?$`).ReplaceAllString(infile, `.2.fasta$1`) - todo <- func() error { - defer phases.Done() - log.Printf("%s starting", infile2) - defer log.Printf("%s done", infile2) - tseqs, err := cmd.tileFasta(tilelib, infile2) - variants[1] = tseqs.Variants() - return err - } - } else { - for phase := 0; phase < 2; phase++ { - phase := phase - todo <- func() error { - defer phases.Done() - log.Printf("%s phase %d starting", infile, phase+1) - defer log.Printf("%s phase %d done", infile, phase+1) - tseqs, err := cmd.tileGVCF(tilelib, infile, phase) - variants[phase] = tseqs.Variants() - return err - } - } - } - encodeJobs.Add(1) - go func() { - defer encodeJobs.Done() - phases.Wait() - if len(errs) > 0 { - return - } - ntags := len(variants[0]) - if ntags < len(variants[1]) { - ntags = len(variants[1]) - } - flat := make([]tileVariantID, ntags*2) - for i := 0; i < ntags; i++ { - for hap := 0; hap < 2; hap++ { - if i < len(variants[hap]) { - flat[i*2+hap] = variants[hap][i] - } - } - } - err := cmd.encoder.Encode(LibraryEntry{ - CompactGenomes: []CompactGenome{{Name: infile, Variants: flat}}, - }) - if err != nil { - select { - case errs <- err: - default: - } - } - }() - } - go close(todo) - var tileJobs sync.WaitGroup - var running int64 - for i := 0; i < runtime.NumCPU()*9/8+1; i++ { - tileJobs.Add(1) - atomic.AddInt64(&running, 1) - go func() { - defer tileJobs.Done() - defer atomic.AddInt64(&running, -1) - for fn := range todo { - if len(errs) > 0 { - return - } - err := fn() - if err != nil { - select { - case errs <- err: - default: - } - } - remain := len(todo) + int(atomic.LoadInt64(&running)) - 1 - ttl := time.Now().Sub(starttime) * time.Duration(remain) / time.Duration(cap(todo)-remain) - eta := time.Now().Add(ttl) - log.Printf("progress %d/%d, eta %v (%v)", cap(todo)-remain, cap(todo), eta, ttl) - } - }() - } - tileJobs.Wait() - encodeJobs.Wait() - go close(errs) - return <-errs -} - -func (cmd *importer) tileGVCF(tilelib *tileLibrary, infile string, phase int) (tileseq tileSeq, err error) { - if cmd.refFile == "" { - err = errors.New("cannot import vcf: reference data (-ref) not specified") - return - } - args := []string{"bcftools", "consensus", "--fasta-ref", cmd.refFile, "-H", fmt.Sprint(phase + 1), infile} - indexsuffix := ".tbi" - if _, err := os.Stat(infile + ".csi"); err == nil { - indexsuffix = ".csi" - } - if out, err := exec.Command("docker", "image", "ls", "-q", "lightning-runtime").Output(); err == nil && len(out) > 0 { - args = append([]string{ - "docker", "run", "--rm", - "--log-driver=none", - "--volume=" + infile + ":" + infile + ":ro", - "--volume=" + infile + indexsuffix + ":" + infile + indexsuffix + ":ro", - "--volume=" + cmd.refFile + ":" + cmd.refFile + ":ro", - "lightning-runtime", - }, args...) - } - consensus := exec.Command(args[0], args[1:]...) - consensus.Stderr = os.Stderr - stdout, err := consensus.StdoutPipe() - defer stdout.Close() - if err != nil { - return - } - err = consensus.Start() - if err != nil { - return - } - defer consensus.Wait() - tileseq, err = tilelib.TileFasta(fmt.Sprintf("%s phase %d", infile, phase+1), stdout) - if err != nil { - return - } - err = stdout.Close() - if err != nil { - return - } - err = consensus.Wait() - if err != nil { - err = fmt.Errorf("%s phase %d: bcftools: %s", infile, phase, err) - return - } - return -}