- log.Infof("seq %s len(refseq) %d len(tilestart) %d", seqname, len(refseq), len(tilestart))
- for tag, tvs := range tiles {
- tag := tagID(tag)
- refstart, ok := tilestart[tag]
- if !ok {
- // Tag didn't place on this
- // reference sequence. (It
- // might place on the same
- // chromosome in a genome
- // anyway, but we don't output
- // the annotations that would
- // result.)
- continue
- }
- for variant, tv := range tvs {
- variant, tv := variant, tv
- if variant == 0 {
- continue
- }
- if len(tv.Sequence) < taglen {
- return fmt.Errorf("tilevar %d,%d has sequence len %d < taglen %d", tag, variant, len(tv.Sequence), taglen)
- }
- var refpart []byte
- endtag := string(tv.Sequence[len(tv.Sequence)-taglen:])
- if endtagid, ok := tag2tagid[endtag]; !ok {
- // Tile variant doesn't end on a tag, so it can only place at the end of a chromosome.
- refpart = refseq[refstart:]
- log.Warnf("%x tilevar %d,%d endtag not in ref: %s", tv.Blake2b[:13], tag, variant, endtag)
- } else if refendtagstart, ok := tilestart[endtagid]; !ok {
- // Ref ends a chromsome with a (possibly very large) variant of this tile, but genomes with this tile don't.
- // Give up. (TODO: something smarter)
- log.Debugf("%x not annotating tilevar %d,%d because end tag %d is not in ref", tv.Blake2b[:13], tag, variant, endtagid)
- continue
+ go func() {
+ defer throttle.Release()
+ throttle.Report(cmd.annotateSequence(throttle, outch, tilelib, taglen, refname, seqname, refcs[seqname], len(refs) > 1))
+ }()
+ }
+ }
+ throttle.Wait()
+ return throttle.Err()
+}
+
+func (cmd *annotatecmd) annotateSequence(throttle *throttle, outch chan<- string, tilelib *tileLibrary, taglen int, refname, seqname string, reftiles []tileLibRef, refnamecol bool) error {
+ refnamefield := ""
+ if refnamecol {
+ refnamefield = "," + trimFilenameForLabel(refname)
+ }
+ var refseq []byte
+ // tilestart[123] is the index into refseq
+ // where the tile for tag 123 was placed.
+ tilestart := map[tagID]int{}
+ tileend := map[tagID]int{}
+ for _, libref := range reftiles {
+ if libref.Variant < 1 {
+ return fmt.Errorf("reference %q seq %q uses variant zero at tag %d", refname, seqname, libref.Tag)
+ }
+ seq := tilelib.TileVariantSequence(libref)
+ if len(seq) < taglen {
+ return fmt.Errorf("reference %q seq %q uses tile %d variant %d with sequence len %d < taglen %d", refname, seqname, libref.Tag, libref.Variant, len(seq), taglen)
+ }
+ overlap := taglen
+ if len(refseq) == 0 {
+ overlap = 0
+ }
+ tilestart[libref.Tag] = len(refseq) - overlap
+ refseq = append(refseq, seq[overlap:]...)
+ tileend[libref.Tag] = len(refseq)
+ }
+ log.Infof("seq %s len(refseq) %d len(tilestart) %d", seqname, len(refseq), len(tilestart))
+ // outtag is tag's index in the subset of tags that aren't
+ // dropped. If there are 10M tags and half are dropped by
+ // dropTiles, tag ranges from 0 to 10M-1 and outtag ranges
+ // from 0 to 5M-1.
+ //
+ // IOW, in the matrix built by cgs2array(), {tag} is
+ // represented by columns {outtag}*2 and {outtag}*2+1.
+ outcol := -1
+ for tag, tvs := range tilelib.variant {
+ if len(cmd.dropTiles) > tag && cmd.dropTiles[tag] {
+ continue
+ }
+ tag := tagID(tag)
+ outcol++
+ // Must shadow outcol var to use safely in goroutine below.
+ outcol := outcol
+ refstart, ok := tilestart[tag]
+ if !ok {
+ // Tag didn't place on this reference
+ // sequence. (It might place on the same
+ // chromosome in a genome anyway, but we don't
+ // output the annotations that would result.)
+ // outch <- fmt.Sprintf("%d,%d,-1%s\n", tag, outcol, refnamefield)
+ continue
+ }
+ for variant := 1; variant <= len(tvs); variant++ {
+ variant, hash := tileVariantID(variant), tvs[variant-1]
+ tileseq := tilelib.TileVariantSequence(tileLibRef{Tag: tag, Variant: variant})
+ if len(tileseq) == 0 {
+ continue
+ } else if len(tileseq) < taglen {
+ return fmt.Errorf("tilevar %d,%d has sequence len %d < taglen %d", tag, variant, len(tileseq), taglen)
+ }
+ var refpart []byte
+ endtag := string(tileseq[len(tileseq)-taglen:])
+ if endtagid, ok := cmd.tag2tagid[endtag]; !ok {
+ // Tile variant doesn't end on a tag, so it can only place at the end of a chromosome.
+ refpart = refseq[refstart:]
+ log.Warnf("%x tilevar %d,%d endtag not in ref: %s", hash[:13], tag, variant, endtag)
+ } else if refendtagstart, ok := tilestart[endtagid]; !ok {
+ // Ref ends a chromsome with a (possibly very large) variant of this tile, but genomes with this tile don't.
+ // Give up. (TODO: something smarter)
+ log.Debugf("%x not annotating tilevar %d,%d because end tag %d is not in ref", hash[:13], tag, variant, endtagid)
+ continue
+ } else {
+ // Non-terminal tile vs. non-terminal reference.
+ refpart = refseq[refstart : refendtagstart+taglen]
+ log.Tracef("\n%x tilevar %d,%d endtag %s endtagid %d refendtagstart %d", hash[:13], tag, variant, endtag, endtagid, refendtagstart)
+ }
+ if len(refpart) > cmd.maxTileSize {
+ log.Warnf("%x tilevar %d,%d skipping long diff, ref %s seq %s pos %d ref len %d", hash[:13], tag, variant, refname, seqname, refstart, len(refpart))
+ continue
+ }
+ if len(tileseq) > cmd.maxTileSize {
+ log.Warnf("%x tilevar %d,%d skipping long diff, ref %s seq %s variant len %d", hash[:13], tag, variant, refname, seqname, len(tileseq))
+ continue
+ }
+ // log.Printf("\n%x @ refstart %d \n< %s\n> %s\n", tv.Blake2b, refstart, refpart, tileseq)
+
+ throttle.Acquire()
+ go func() {
+ defer throttle.Release()
+ diffs, _ := hgvs.Diff(strings.ToUpper(string(refpart)), strings.ToUpper(string(tileseq)), 0)
+ for _, diff := range diffs {
+ diff.Position += refstart
+ var varid string
+ if cmd.variantHash {
+ varid = fmt.Sprintf("%x", hash)[:13]