+ retainNoCalls bool
+ skipOOO bool
+ retainTileSequences bool
+ useDups bool
+
+ taglib *tagLibrary
+ variant [][][blake2b.Size256]byte
+ refseqs map[string]map[string][]tileLibRef
+ compactGenomes map[string][]tileVariantID
+ seq2 map[[2]byte]map[[blake2b.Size256]byte][]byte
+ seq2lock map[[2]byte]sync.Locker
+ variants int64
+ // if non-nil, write out any tile variants added while tiling
+ encoder *gob.Encoder
+ // set Ref flag when writing new variants to encoder
+ encodeRef bool
+
+ onAddTileVariant func(libref tileLibRef, hash [blake2b.Size256]byte, seq []byte) error
+ onAddGenome func(CompactGenome) error
+ onAddRefseq func(CompactSequence) error
+
+ mtx sync.RWMutex
+ vlock []sync.Locker
+}
+
+func (tilelib *tileLibrary) loadTagSet(newtagset [][]byte) error {
+ // Loading a tagset means either passing it through to the
+ // output (if it's the first one we've seen), or just ensuring
+ // it doesn't disagree with what we already have.
+ if len(newtagset) == 0 {
+ return nil
+ }
+ tilelib.mtx.Lock()
+ defer tilelib.mtx.Unlock()
+ if tilelib.taglib == nil || tilelib.taglib.Len() == 0 {
+ tilelib.taglib = &tagLibrary{}
+ err := tilelib.taglib.setTags(newtagset)
+ if err != nil {
+ return err
+ }
+ if tilelib.encoder != nil {
+ err = tilelib.encoder.Encode(LibraryEntry{
+ TagSet: newtagset,
+ })
+ if err != nil {
+ return err
+ }
+ }
+ } else if tilelib.taglib.Len() != len(newtagset) {
+ return fmt.Errorf("cannot merge libraries with differing tagsets")
+ } else {
+ current := tilelib.taglib.Tags()
+ for i := range newtagset {
+ if !bytes.Equal(newtagset[i], current[i]) {
+ return fmt.Errorf("cannot merge libraries with differing tagsets")
+ }
+ }
+ }
+ return nil
+}
+
+func (tilelib *tileLibrary) loadTileVariants(tvs []TileVariant, variantmap map[tileLibRef]tileVariantID) error {
+ for _, tv := range tvs {
+ // Assign a new variant ID (unique across all inputs)
+ // for each input variant.
+ variantmap[tileLibRef{Tag: tv.Tag, Variant: tv.Variant}] = tilelib.getRef(tv.Tag, tv.Sequence, tv.Ref).Variant
+ }
+ return nil
+}
+
+func (tilelib *tileLibrary) loadCompactGenomes(cgs []CompactGenome, variantmap map[tileLibRef]tileVariantID) error {
+ log.Debugf("loadCompactGenomes: %d", len(cgs))
+ var wg sync.WaitGroup
+ errs := make(chan error, 1)
+ for _, cg := range cgs {
+ wg.Add(1)
+ cg := cg
+ go func() {
+ defer wg.Done()
+ for i, variant := range cg.Variants {
+ if len(errs) > 0 {
+ return
+ }
+ if variant == 0 {
+ continue
+ }
+ tag := tagID(i / 2)
+ newvariant, ok := variantmap[tileLibRef{Tag: tag, Variant: variant}]
+ if !ok {
+ err := fmt.Errorf("oops: genome %q has variant %d for tag %d, but that variant was not in its library", cg.Name, variant, tag)
+ select {
+ case errs <- err:
+ default:
+ }
+ return
+ }
+ // log.Tracef("loadCompactGenomes: cg %s tag %d variant %d => %d", cg.Name, tag, variant, newvariant)
+ cg.Variants[i] = newvariant
+ }
+ if tilelib.onAddGenome != nil {
+ err := tilelib.onAddGenome(cg)
+ if err != nil {
+ select {
+ case errs <- err:
+ default:
+ }
+ return
+ }
+ }
+ if tilelib.encoder != nil {
+ err := tilelib.encoder.Encode(LibraryEntry{
+ CompactGenomes: []CompactGenome{cg},
+ })
+ if err != nil {
+ select {
+ case errs <- err:
+ default:
+ }
+ return
+ }
+ }
+ if tilelib.compactGenomes != nil {
+ tilelib.mtx.Lock()
+ defer tilelib.mtx.Unlock()
+ tilelib.compactGenomes[cg.Name] = cg.Variants
+ }
+ }()
+ }
+ wg.Wait()
+ go close(errs)
+ return <-errs
+}
+
+func (tilelib *tileLibrary) loadCompactSequences(cseqs []CompactSequence, variantmap map[tileLibRef]tileVariantID) error {
+ log.Infof("loadCompactSequences: %d todo", len(cseqs))
+ for _, cseq := range cseqs {
+ log.Infof("loadCompactSequences: checking %s", cseq.Name)
+ for _, tseq := range cseq.TileSequences {
+ for i, libref := range tseq {
+ if libref.Variant == 0 {
+ // No variant (e.g., import
+ // dropped tiles with
+ // no-calls) = no translation.
+ continue
+ }
+ v, ok := variantmap[libref]
+ if !ok {
+ return fmt.Errorf("oops: CompactSequence %q has variant %d for tag %d, but that variant was not in its library", cseq.Name, libref.Variant, libref.Tag)
+ }
+ tseq[i].Variant = v
+ }
+ }
+ if tilelib.encoder != nil {
+ if err := tilelib.encoder.Encode(LibraryEntry{
+ CompactSequences: []CompactSequence{cseq},
+ }); err != nil {
+ return err
+ }
+ }
+ if tilelib.onAddRefseq != nil {
+ err := tilelib.onAddRefseq(cseq)
+ if err != nil {
+ return err
+ }
+ }
+ log.Infof("loadCompactSequences: checking %s done", cseq.Name)
+ }
+ tilelib.mtx.Lock()
+ defer tilelib.mtx.Unlock()
+ if tilelib.refseqs == nil {
+ tilelib.refseqs = map[string]map[string][]tileLibRef{}
+ }
+ for _, cseq := range cseqs {
+ tilelib.refseqs[cseq.Name] = cseq.TileSequences
+ }
+ log.Info("loadCompactSequences: done")
+ return nil
+}
+
+func allFiles(path string, re *regexp.Regexp) ([]string, error) {
+ var files []string
+ f, err := open(path)
+ if err != nil {
+ return nil, err
+ }
+ defer f.Close()
+ fis, err := f.Readdir(-1)
+ if err != nil {
+ return []string{path}, nil
+ }
+ for _, fi := range fis {
+ if fi.Name() == "." || fi.Name() == ".." {
+ continue
+ } else if child := path + "/" + fi.Name(); fi.IsDir() {
+ add, err := allFiles(child, re)
+ if err != nil {
+ return nil, err
+ }
+ files = append(files, add...)
+ } else if re == nil || re.MatchString(child) {
+ files = append(files, child)
+ }
+ }
+ sort.Strings(files)
+ return files, nil
+}
+
+var matchGobFile = regexp.MustCompile(`\.gob(\.gz)?$`)
+
+func (tilelib *tileLibrary) LoadDir(ctx context.Context, path string) error {
+ log.Infof("LoadDir: walk dir %s", path)
+ files, err := allFiles(path, matchGobFile)
+ if err != nil {
+ return err
+ }
+ ctx, cancel := context.WithCancel(ctx)
+ defer cancel()
+ var mtx sync.Mutex
+ allcgs := make([][]CompactGenome, len(files))
+ allcseqs := make([][]CompactSequence, len(files))
+ allvariantmap := map[tileLibRef]tileVariantID{}
+ errs := make(chan error, len(files))
+ log.Infof("LoadDir: read %d files", len(files))
+ for fileno, path := range files {
+ fileno, path := fileno, path
+ go func() {
+ f, err := open(path)
+ if err != nil {
+ errs <- err
+ return
+ }
+ defer f.Close()
+ defer log.Infof("LoadDir: finished reading %s", path)
+
+ var variantmap = map[tileLibRef]tileVariantID{}
+ var cgs []CompactGenome
+ var cseqs []CompactSequence
+ err = DecodeLibrary(f, strings.HasSuffix(path, ".gz"), func(ent *LibraryEntry) error {
+ if ctx.Err() != nil {
+ return ctx.Err()
+ }
+ if len(ent.TagSet) > 0 {
+ mtx.Lock()
+ if tilelib.taglib == nil || tilelib.taglib.Len() != len(ent.TagSet) {
+ // load first set of tags, or
+ // report mismatch if 2 sets
+ // have different #tags.
+ if err := tilelib.loadTagSet(ent.TagSet); err != nil {
+ mtx.Unlock()
+ return err
+ }
+ }
+ mtx.Unlock()
+ }
+ for _, tv := range ent.TileVariants {
+ variantmap[tileLibRef{Tag: tv.Tag, Variant: tv.Variant}] = tilelib.getRef(tv.Tag, tv.Sequence, tv.Ref).Variant
+ }
+ cgs = append(cgs, ent.CompactGenomes...)
+ cseqs = append(cseqs, ent.CompactSequences...)
+ return nil
+ })
+ allcgs[fileno] = cgs
+ allcseqs[fileno] = cseqs
+ mtx.Lock()
+ defer mtx.Unlock()
+ for k, v := range variantmap {
+ allvariantmap[k] = v
+ }
+ errs <- err
+ }()
+ }
+ for range files {
+ err := <-errs
+ if err != nil {
+ return err
+ }
+ }
+
+ log.Info("LoadDir: loadCompactGenomes")
+ var flatcgs []CompactGenome
+ for _, cgs := range allcgs {
+ flatcgs = append(flatcgs, cgs...)
+ }
+ err = tilelib.loadCompactGenomes(flatcgs, allvariantmap)
+ if err != nil {
+ return err
+ }
+
+ log.Info("LoadDir: loadCompactSequences")
+ var flatcseqs []CompactSequence
+ for _, cseqs := range allcseqs {
+ flatcseqs = append(flatcseqs, cseqs...)
+ }
+ err = tilelib.loadCompactSequences(flatcseqs, allvariantmap)
+ if err != nil {
+ return err
+ }
+
+ log.Info("LoadDir done")
+ return nil
+}
+
+func (tilelib *tileLibrary) WriteDir(dir string) error {
+ ntilefiles := 128
+ nfiles := ntilefiles + len(tilelib.refseqs)
+ files := make([]*os.File, nfiles)
+ for i := range files {
+ f, err := os.OpenFile(fmt.Sprintf("%s/library.%04d.gob.gz", dir, i), os.O_CREATE|os.O_WRONLY, 0666)
+ if err != nil {
+ return err
+ }
+ defer f.Close()
+ files[i] = f
+ }
+ bufws := make([]*bufio.Writer, nfiles)
+ for i := range bufws {
+ bufws[i] = bufio.NewWriterSize(files[i], 1<<26)
+ }
+ zws := make([]*pgzip.Writer, nfiles)
+ for i := range zws {
+ zws[i] = pgzip.NewWriter(bufws[i])
+ defer zws[i].Close()
+ }
+ encoders := make([]*gob.Encoder, nfiles)
+ for i := range encoders {
+ encoders[i] = gob.NewEncoder(zws[i])
+ }
+
+ cgnames := make([]string, 0, len(tilelib.compactGenomes))
+ for name := range tilelib.compactGenomes {
+ cgnames = append(cgnames, name)
+ }
+ sort.Strings(cgnames)
+
+ refnames := make([]string, 0, len(tilelib.refseqs))
+ for name := range tilelib.refseqs {
+ refnames = append(refnames, name)
+ }
+ sort.Strings(refnames)
+
+ log.Infof("WriteDir: writing %d files", nfiles)
+ ctx, cancel := context.WithCancel(context.Background())
+ defer cancel()
+ errs := make(chan error, nfiles)
+ for start := range files {
+ start := start
+ go func() {
+ err := encoders[start].Encode(LibraryEntry{TagSet: tilelib.taglib.Tags()})
+ if err != nil {
+ errs <- err
+ return
+ }
+ if refidx := start - ntilefiles; refidx >= 0 {
+ // write each ref to its own file
+ // (they seem to load very slowly)
+ name := refnames[refidx]
+ errs <- encoders[start].Encode(LibraryEntry{CompactSequences: []CompactSequence{{
+ Name: name,
+ TileSequences: tilelib.refseqs[name],
+ }}})
+ return
+ }
+ for i := start; i < len(cgnames); i += ntilefiles {
+ err := encoders[start].Encode(LibraryEntry{CompactGenomes: []CompactGenome{{
+ Name: cgnames[i],
+ Variants: tilelib.compactGenomes[cgnames[i]],
+ }}})
+ if err != nil {
+ errs <- err
+ return
+ }
+ }
+ tvs := []TileVariant{}
+ for tag := start; tag < len(tilelib.variant) && ctx.Err() == nil; tag += ntilefiles {
+ tvs = tvs[:0]
+ for idx, hash := range tilelib.variant[tag] {
+ tvs = append(tvs, TileVariant{
+ Tag: tagID(tag),
+ Variant: tileVariantID(idx + 1),
+ Blake2b: hash,
+ Sequence: tilelib.hashSequence(hash),
+ })
+ }
+ err := encoders[start].Encode(LibraryEntry{TileVariants: tvs})
+ if err != nil {
+ errs <- err
+ return
+ }
+ }
+ errs <- nil
+ }()
+ }
+ for range files {
+ err := <-errs
+ if err != nil {
+ return err
+ }
+ }
+ log.Info("WriteDir: flushing")
+ for i := range zws {
+ err := zws[i].Close()
+ if err != nil {
+ return err
+ }
+ err = bufws[i].Flush()
+ if err != nil {
+ return err
+ }
+ err = files[i].Close()
+ if err != nil {
+ return err
+ }
+ }
+ log.Info("WriteDir: done")
+ return nil
+}
+
+// Load library data from rdr. Tile variants might be renumbered in
+// the process; in that case, genomes variants will be renumbered to
+// match.
+func (tilelib *tileLibrary) LoadGob(ctx context.Context, rdr io.Reader, gz bool) error {
+ cgs := []CompactGenome{}
+ cseqs := []CompactSequence{}
+ variantmap := map[tileLibRef]tileVariantID{}
+ err := DecodeLibrary(rdr, gz, func(ent *LibraryEntry) error {
+ if ctx.Err() != nil {
+ return ctx.Err()
+ }
+ if err := tilelib.loadTagSet(ent.TagSet); err != nil {
+ return err
+ }
+ if err := tilelib.loadTileVariants(ent.TileVariants, variantmap); err != nil {
+ return err
+ }
+ cgs = append(cgs, ent.CompactGenomes...)
+ cseqs = append(cseqs, ent.CompactSequences...)
+ return nil
+ })
+ if err != nil {
+ return err
+ }
+ if ctx.Err() != nil {
+ return ctx.Err()
+ }
+ err = tilelib.loadCompactGenomes(cgs, variantmap)
+ if err != nil {
+ return err
+ }
+ err = tilelib.loadCompactSequences(cseqs, variantmap)
+ if err != nil {
+ return err
+ }
+ return nil
+}
+
+func (tilelib *tileLibrary) dump(out io.Writer) {
+ printTV := func(tag int, variant tileVariantID) {
+ if variant < 1 {
+ fmt.Fprintf(out, " -")
+ } else if tag >= len(tilelib.variant) {
+ fmt.Fprintf(out, " (!tag=%d)", tag)
+ } else if int(variant) > len(tilelib.variant[tag]) {
+ fmt.Fprintf(out, " (tag=%d,!variant=%d)", tag, variant)
+ } else {
+ fmt.Fprintf(out, " %x", tilelib.variant[tag][variant-1][:8])
+ }
+ }
+ for refname, refseqs := range tilelib.refseqs {
+ for seqname, seq := range refseqs {
+ fmt.Fprintf(out, "ref %s %s", refname, seqname)
+ for _, libref := range seq {
+ printTV(int(libref.Tag), libref.Variant)
+ }
+ fmt.Fprintf(out, "\n")
+ }
+ }
+ for name, cg := range tilelib.compactGenomes {
+ fmt.Fprintf(out, "cg %s", name)
+ for tag, variant := range cg {
+ printTV(tag/2, variant)
+ }
+ fmt.Fprintf(out, "\n")
+ }
+}