import (
"bufio"
"bytes"
+ "context"
"encoding/gob"
+ "fmt"
"io"
"strings"
"sync"
type tileVariantID uint16 // 1-based
type tileLibRef struct {
- tag tagID
- variant tileVariantID
+ Tag tagID
+ Variant tileVariantID
}
type tileSeq map[string][]tileLibRef
maxtag := 0
for _, refs := range tseq {
for _, ref := range refs {
- if maxtag < int(ref.tag) {
- maxtag = int(ref.tag)
+ if maxtag < int(ref.Tag) {
+ maxtag = int(ref.Tag)
}
}
}
var kept, dropped int
for _, refs := range tseq {
for _, ref := range refs {
- if vars[int(ref.tag)] != 0 {
+ if vars[int(ref.Tag)] != 0 {
dropped++
} else {
kept++
}
- vars[int(ref.tag)] = ref.variant
+ vars[int(ref.Tag)] = ref.Variant
}
}
return vars, kept, dropped
skipOOO bool
taglib *tagLibrary
variant [][][blake2b.Size256]byte
+ refseqs map[string]map[string][]tileLibRef
// count [][]int
// seq map[[blake2b.Size]byte][]byte
variants int
mtx sync.Mutex
}
-func (tilelib *tileLibrary) TileFasta(filelabel string, rdr io.Reader) (tileSeq, error) {
+func (tilelib *tileLibrary) loadTagSet(newtagset [][]byte) error {
+ // Loading a tagset means either passing it through to the
+ // output (if it's the first one we've seen), or just ensuring
+ // it doesn't disagree with what we already have.
+ if len(newtagset) == 0 {
+ return nil
+ }
+ tilelib.mtx.Lock()
+ defer tilelib.mtx.Unlock()
+ if tilelib.taglib == nil || tilelib.taglib.Len() == 0 {
+ tilelib.taglib = &tagLibrary{}
+ err := tilelib.taglib.setTags(newtagset)
+ if err != nil {
+ return err
+ }
+ if tilelib.encoder != nil {
+ err = tilelib.encoder.Encode(LibraryEntry{
+ TagSet: newtagset,
+ })
+ if err != nil {
+ return err
+ }
+ }
+ } else if tilelib.taglib.Len() != len(newtagset) {
+ return fmt.Errorf("cannot merge libraries with differing tagsets")
+ } else {
+ current := tilelib.taglib.Tags()
+ for i := range newtagset {
+ if !bytes.Equal(newtagset[i], current[i]) {
+ return fmt.Errorf("cannot merge libraries with differing tagsets")
+ }
+ }
+ }
+ return nil
+}
+
+func (tilelib *tileLibrary) loadTileVariants(tvs []TileVariant, variantmap map[tileLibRef]tileVariantID) error {
+ for _, tv := range tvs {
+ // Assign a new variant ID (unique across all inputs)
+ // for each input variant.
+ variantmap[tileLibRef{Tag: tv.Tag, Variant: tv.Variant}] = tilelib.getRef(tv.Tag, tv.Sequence).Variant
+ }
+ return nil
+}
+
+func (tilelib *tileLibrary) loadCompactGenomes(cgs []CompactGenome, variantmap map[tileLibRef]tileVariantID, onLoadGenome func(CompactGenome)) error {
+ log.Debugf("loadCompactGenomes: %d", len(cgs))
+ var wg sync.WaitGroup
+ errs := make(chan error, 1)
+ for _, cg := range cgs {
+ wg.Add(1)
+ cg := cg
+ go func() {
+ defer wg.Done()
+ for i, variant := range cg.Variants {
+ if len(errs) > 0 {
+ return
+ }
+ if variant == 0 {
+ continue
+ }
+ tag := tagID(i / 2)
+ newvariant, ok := variantmap[tileLibRef{Tag: tag, Variant: variant}]
+ if !ok {
+ err := fmt.Errorf("oops: genome %q has variant %d for tag %d, but that variant was not in its library", cg.Name, variant, tag)
+ select {
+ case errs <- err:
+ default:
+ }
+ return
+ }
+ log.Tracef("loadCompactGenomes: cg %s tag %d variant %d => %d", cg.Name, tag, variant, newvariant)
+ cg.Variants[i] = newvariant
+ }
+ if onLoadGenome != nil {
+ onLoadGenome(cg)
+ }
+ if tilelib.encoder != nil {
+ err := tilelib.encoder.Encode(LibraryEntry{
+ CompactGenomes: []CompactGenome{cg},
+ })
+ if err != nil {
+ select {
+ case errs <- err:
+ default:
+ }
+ return
+ }
+ }
+ }()
+ }
+ wg.Wait()
+ go close(errs)
+ return <-errs
+}
+
+func (tilelib *tileLibrary) loadCompactSequences(cseqs []CompactSequence, variantmap map[tileLibRef]tileVariantID) error {
+ log.Debugf("loadCompactSequences: %d", len(cseqs))
+ for _, cseq := range cseqs {
+ for _, tseq := range cseq.TileSequences {
+ for i, libref := range tseq {
+ if libref.Variant == 0 {
+ // No variant (e.g., import
+ // dropped tiles with
+ // no-calls) = no translation.
+ continue
+ }
+ v, ok := variantmap[libref]
+ if !ok {
+ return fmt.Errorf("oops: CompactSequence %q has variant %d for tag %d, but that variant was not in its library", cseq.Name, libref.Variant, libref.Tag)
+ }
+ tseq[i].Variant = v
+ }
+ }
+ if tilelib.encoder != nil {
+ if err := tilelib.encoder.Encode(LibraryEntry{
+ CompactSequences: []CompactSequence{cseq},
+ }); err != nil {
+ return err
+ }
+ }
+ }
+ tilelib.mtx.Lock()
+ defer tilelib.mtx.Unlock()
+ if tilelib.refseqs == nil {
+ tilelib.refseqs = map[string]map[string][]tileLibRef{}
+ }
+ for _, cseq := range cseqs {
+ tilelib.refseqs[cseq.Name] = cseq.TileSequences
+ }
+ return nil
+}
+
+// Load library data from rdr. Tile variants might be renumbered in
+// the process; in that case, genomes variants will be renumbered to
+// match.
+//
+// If onLoadGenome is non-nil, call it on each CompactGenome entry.
+func (tilelib *tileLibrary) LoadGob(ctx context.Context, rdr io.Reader, onLoadGenome func(CompactGenome)) error {
+ cgs := []CompactGenome{}
+ cseqs := []CompactSequence{}
+ variantmap := map[tileLibRef]tileVariantID{}
+ err := DecodeLibrary(rdr, func(ent *LibraryEntry) error {
+ if ctx.Err() != nil {
+ return ctx.Err()
+ }
+ if err := tilelib.loadTagSet(ent.TagSet); err != nil {
+ return err
+ }
+ if err := tilelib.loadTileVariants(ent.TileVariants, variantmap); err != nil {
+ return err
+ }
+ cgs = append(cgs, ent.CompactGenomes...)
+ cseqs = append(cseqs, ent.CompactSequences...)
+ return nil
+ })
+ if err != nil {
+ return err
+ }
+ if ctx.Err() != nil {
+ return ctx.Err()
+ }
+ err = tilelib.loadCompactGenomes(cgs, variantmap, onLoadGenome)
+ if err != nil {
+ return err
+ }
+ err = tilelib.loadCompactSequences(cseqs, variantmap)
+ if err != nil {
+ return err
+ }
+ return nil
+}
+
+type importStats struct {
+ InputFile string
+ InputLabel string
+ InputLength int
+ InputCoverage int
+ TileCoverage int
+ PathLength int
+ DroppedOutOfOrderTiles int
+}
+
+func (tilelib *tileLibrary) TileFasta(filelabel string, rdr io.Reader) (tileSeq, []importStats, error) {
ret := tileSeq{}
type jobT struct {
label string
buf := scanner.Bytes()
if len(buf) > 0 && buf[0] == '>' {
todo <- jobT{seqlabel, fasta}
- seqlabel, fasta = string(buf[1:]), nil
+ seqlabel, fasta = strings.SplitN(string(buf[1:]), " ", 2)[0], nil
log.Debugf("%s %s reading fasta", filelabel, seqlabel)
} else {
fasta = append(fasta, bytes.ToLower(buf)...)
totalFoundTags := 0
totalPathLen := 0
skippedSequences := 0
+ stats := make([]importStats, 0, len(todo))
for job := range todo {
if len(job.fasta) == 0 {
continue
})
totalFoundTags += len(found)
+ basesOut := 0
skipped := 0
path = path[:0]
last := foundtag{tagid: -1}
}
for i, f := range found {
log.Tracef("%s %s found[%d] == %#v", filelabel, job.label, i, f)
- if last.taglen > 0 {
- path = append(path, tilelib.getRef(last.tagid, job.fasta[last.pos:f.pos+f.taglen]))
+ if last.tagid < 0 {
+ // first tag in sequence
+ last = foundtag{tagid: f.tagid}
+ continue
+ }
+ libref := tilelib.getRef(last.tagid, job.fasta[last.pos:f.pos+f.taglen])
+ path = append(path, libref)
+ if libref.Variant > 0 {
+ // Count output coverage from
+ // the end of the previous tag
+ // (if any) to the end of the
+ // current tag, IOW don't
+ // double-count coverage for
+ // the previous tag.
+ basesOut += countBases(job.fasta[last.pos+last.taglen : f.pos+f.taglen])
+ } else {
+ // If we dropped this tile
+ // (because !includeNoCalls),
+ // set taglen=0 so the
+ // overlapping tag is counted
+ // toward coverage on the
+ // following tile.
+ f.taglen = 0
}
last = f
}
- if last.taglen > 0 {
- path = append(path, tilelib.getRef(last.tagid, job.fasta[last.pos:]))
+ if last.tagid < 0 {
+ log.Warnf("%s %s no tags found", filelabel, job.label)
+ } else {
+ libref := tilelib.getRef(last.tagid, job.fasta[last.pos:])
+ path = append(path, libref)
+ if libref.Variant > 0 {
+ basesOut += countBases(job.fasta[last.pos+last.taglen:])
+ }
}
pathcopy := make([]tileLibRef, len(path))
copy(pathcopy, path)
ret[job.label] = pathcopy
log.Debugf("%s %s tiled with path len %d, skipped %d", filelabel, job.label, len(path), skipped)
+
+ basesIn := countBases(job.fasta)
+ log.Infof("%s %s fasta in %d coverage in %d coverage out %d", filelabel, job.label, len(job.fasta), basesIn, basesOut)
+ stats = append(stats, importStats{
+ InputFile: filelabel,
+ InputLabel: job.label,
+ InputLength: len(job.fasta),
+ InputCoverage: basesIn,
+ TileCoverage: basesOut,
+ PathLength: len(path),
+ DroppedOutOfOrderTiles: skipped,
+ })
+
totalPathLen += len(path)
}
log.Printf("%s tiled with total path len %d in %d sequences (skipped %d sequences with '_' in name, skipped %d out-of-order tags)", filelabel, totalPathLen, len(ret), skippedSequences, totalFoundTags-totalPathLen)
- return ret, scanner.Err()
+ return ret, stats, scanner.Err()
}
func (tilelib *tileLibrary) Len() int {
if b != 'a' && b != 'c' && b != 'g' && b != 't' {
// return "tile not found" if seq has any
// no-calls
- return tileLibRef{tag: tag}
+ return tileLibRef{Tag: tag}
}
}
}
// if tilelib.seq == nil {
// tilelib.seq = map[[blake2b.Size]byte][]byte{}
// }
- if tilelib.variant == nil {
+ if tilelib.variant == nil && tilelib.taglib != nil {
tilelib.variant = make([][][blake2b.Size256]byte, tilelib.taglib.Len())
}
+ if int(tag) >= len(tilelib.variant) {
+ // If we haven't seen the tag library yet (as in a
+ // merge), tilelib.taglib.Len() is zero. We can still
+ // behave correctly, we just need to expand the
+ // tilelib.variant slice as needed.
+ if int(tag) >= cap(tilelib.variant) {
+ // Allocate 2x capacity.
+ newslice := make([][][blake2b.Size256]byte, int(tag)+1, (int(tag)+1)*2)
+ copy(newslice, tilelib.variant)
+ tilelib.variant = newslice[:int(tag)+1]
+ } else {
+ // Use previously allocated capacity, avoiding
+ // copy.
+ tilelib.variant = tilelib.variant[:int(tag)+1]
+ }
+ }
seqhash := blake2b.Sum256(seq)
for i, varhash := range tilelib.variant[tag] {
if varhash == seqhash {
tilelib.mtx.Unlock()
- return tileLibRef{tag: tag, variant: tileVariantID(i + 1)}
+ return tileLibRef{Tag: tag, Variant: tileVariantID(i + 1)}
}
}
tilelib.variants++
tilelib.variant[tag] = append(tilelib.variant[tag], seqhash)
// tilelib.seq[seqhash] = append([]byte(nil), seq...)
variant := tileVariantID(len(tilelib.variant[tag]))
- ret := tileLibRef{tag: tag, variant: variant}
tilelib.mtx.Unlock()
if tilelib.encoder != nil {
}},
})
}
- return ret
+ return tileLibRef{Tag: tag, Variant: variant}
+}
+
+func countBases(seq []byte) int {
+ n := 0
+ for _, c := range seq {
+ if isbase[c] {
+ n++
+ }
+ }
+ return n
}
projects / lightning.git / blobdiff