// Copyright (C) The Lightning Authors. All rights reserved. // // SPDX-License-Identifier: AGPL-3.0 package lightning import ( "bufio" "bytes" "flag" "fmt" "io" "net/http" _ "net/http/pprof" "os" "regexp" "runtime" "sort" "strconv" "strings" "sync" "sync/atomic" "git.arvados.org/arvados.git/sdk/go/arvados" log "github.com/sirupsen/logrus" "golang.org/x/crypto/blake2b" ) type dump struct { filter filter cgnames []string selectedTags map[tagID]bool } func (cmd *dump) RunCommand(prog string, args []string, stdin io.Reader, stdout, stderr io.Writer) int { err := cmd.run(prog, args, stdin, stdout, stderr) if err != nil { return 1 } return 0 } func (cmd *dump) run(prog string, args []string, stdin io.Reader, stdout, stderr io.Writer) error { flags := flag.NewFlagSet("", flag.ContinueOnError) flags.SetOutput(stderr) pprof := flags.String("pprof", "", "serve Go profile data at http://`[addr]:port`") runlocal := flags.Bool("local", false, "run on local host (default: run in an arvados container)") projectUUID := flags.String("project", "", "project `UUID` for output data") priority := flags.Int("priority", 500, "container request priority") inputDir := flags.String("input-dir", "./in", "input `directory`") outputDir := flags.String("output-dir", "./out", "output `directory`") ref := flags.String("ref", "", "reference name (if blank, choose last one that appears in input)") regionsFilename := flags.String("regions", "", "only output columns/annotations that intersect regions in specified bed `file`") expandRegions := flags.Int("expand-regions", 0, "expand specified regions by `N` base pairs on each side`") selectedTags := flags.String("tags", "", "tag numbers to dump") cmd.filter.Flags(flags) err := flags.Parse(args) if err == flag.ErrHelp { return nil } else if err != nil { return err } else if flags.NArg() > 0 { return fmt.Errorf("errant command line arguments after parsed flags: %v", flags.Args()) } if *pprof != "" { go func() { log.Println(http.ListenAndServe(*pprof, nil)) }() } if !*runlocal { runner := arvadosContainerRunner{ Name: "lightning slice-numpy", Client: arvados.NewClientFromEnv(), ProjectUUID: *projectUUID, RAM: 750000000000, VCPUs: 96, Priority: *priority, KeepCache: 2, APIAccess: true, } err = runner.TranslatePaths(inputDir, regionsFilename) if err != nil { return err } runner.Args = []string{"dump", "-local=true", "-pprof=:6060", "-input-dir=" + *inputDir, "-output-dir=/mnt/output", "-regions=" + *regionsFilename, "-expand-regions=" + fmt.Sprintf("%d", *expandRegions), "-tags=" + *selectedTags, } runner.Args = append(runner.Args, cmd.filter.Args()...) output, err := runner.Run() if err != nil { return err } fmt.Fprintln(stdout, output) return nil } if *selectedTags != "" { cmd.selectedTags = map[tagID]bool{} for _, tagstr := range strings.Split(*selectedTags, ",") { tag, err := strconv.ParseInt(tagstr, 10, 64) if err != nil { return err } cmd.selectedTags[tagID(tag)] = true } } infiles, err := allFiles(*inputDir, matchGobFile) if err != nil { return err } if len(infiles) == 0 { return fmt.Errorf("no input files found in %s", *inputDir) } sort.Strings(infiles) var refseq map[string][]tileLibRef var reftiledata = make(map[tileLibRef][]byte, 11000000) in0, err := open(infiles[0]) if err != nil { return err } matchGenome, err := regexp.Compile(cmd.filter.MatchGenome) if err != nil { err = fmt.Errorf("-match-genome: invalid regexp: %q", cmd.filter.MatchGenome) return err } cmd.cgnames = nil taglen := -1 DecodeLibrary(in0, strings.HasSuffix(infiles[0], ".gz"), func(ent *LibraryEntry) error { if len(ent.TagSet) > 0 { taglen = len(ent.TagSet[0]) } for _, cseq := range ent.CompactSequences { if cseq.Name == *ref || *ref == "" { refseq = cseq.TileSequences } } for _, cg := range ent.CompactGenomes { if matchGenome.MatchString(cg.Name) { cmd.cgnames = append(cmd.cgnames, cg.Name) } } for _, tv := range ent.TileVariants { if tv.Ref { reftiledata[tileLibRef{tv.Tag, tv.Variant}] = tv.Sequence } } return nil }) if err != nil { return err } in0.Close() if refseq == nil { return fmt.Errorf("%s: reference sequence not found", infiles[0]) } if taglen < 0 { return fmt.Errorf("tagset not found") } if len(cmd.cgnames) == 0 { return fmt.Errorf("no genomes found matching regexp %q", cmd.filter.MatchGenome) } sort.Strings(cmd.cgnames) log.Info("indexing reference tiles") type reftileinfo struct { variant tileVariantID seqname string // chr1 pos int // distance from start of chromosome to starttag tiledata []byte // acgtggcaa... } isdup := map[tagID]bool{} reftile := map[tagID]*reftileinfo{} for seqname, cseq := range refseq { pos := 0 for _, libref := range cseq { tiledata := reftiledata[libref] if len(tiledata) == 0 { return fmt.Errorf("missing tiledata for tag %d variant %d in %s in ref", libref.Tag, libref.Variant, seqname) } if isdup[libref.Tag] { log.Printf("dropping reference tile %+v from %s @ %d, tag not unique", libref, seqname, pos) } else if reftile[libref.Tag] != nil { log.Printf("dropping reference tile %+v from %s @ %d, tag not unique", tileLibRef{Tag: libref.Tag, Variant: reftile[libref.Tag].variant}, reftile[libref.Tag].seqname, reftile[libref.Tag].pos) delete(reftile, libref.Tag) log.Printf("dropping reference tile %+v from %s @ %d, tag not unique", libref, seqname, pos) isdup[libref.Tag] = true } else { reftile[libref.Tag] = &reftileinfo{ seqname: seqname, variant: libref.Variant, tiledata: tiledata, pos: pos, } } pos += len(tiledata) - taglen } log.Printf("... %s done, len %d", seqname, pos+taglen) } var mask *mask if *regionsFilename != "" { log.Printf("loading regions from %s", *regionsFilename) mask, err = makeMask(*regionsFilename, *expandRegions) if err != nil { return err } log.Printf("before applying mask, len(reftile) == %d", len(reftile)) log.Printf("deleting reftile entries for regions outside %d intervals", mask.Len()) for tag, rt := range reftile { if !mask.Check(strings.TrimPrefix(rt.seqname, "chr"), rt.pos, rt.pos+len(rt.tiledata)) { delete(reftile, tag) } } log.Printf("after applying mask, len(reftile) == %d", len(reftile)) } if cmd.selectedTags != nil { log.Printf("deleting reftile entries other than %d selected tags", len(cmd.selectedTags)) for tag := range reftile { if !cmd.selectedTags[tag] { delete(reftile, tag) } } log.Printf("after applying selected tags, len(reftile) == %d", len(reftile)) } dumpVariantsName := fmt.Sprintf("%s/variants.csv", *outputDir) log.Infof("writing %s", dumpVariantsName) dumpVariantsF, err := os.Create(dumpVariantsName) if err != nil { return err } dumpVariantsW := bufio.NewWriterSize(dumpVariantsF, 1<<20) mtx := sync.Mutex{} throttleMem := throttle{Max: runtime.GOMAXPROCS(0)} log.Infof("reading %d slices with max concurrency %d", len(infiles), throttleMem.Max) var done int64 for infileIdx, infile := range infiles { infileIdx, infile := infileIdx, infile throttleMem.Go(func() error { seq := make(map[tagID][]TileVariant, 50000) cgs := make(map[string]CompactGenome, len(cmd.cgnames)) f, err := open(infile) if err != nil { return err } defer f.Close() log.Infof("%04d: reading %s", infileIdx, infile) err = DecodeLibrary(f, strings.HasSuffix(infile, ".gz"), func(ent *LibraryEntry) error { for _, tv := range ent.TileVariants { if tv.Ref { continue } if mask != nil && reftile[tv.Tag] == nil { // Don't waste // time/memory on // masked-out tiles. continue } variants := seq[tv.Tag] if len(variants) == 0 { variants = make([]TileVariant, 100) } for len(variants) <= int(tv.Variant) { variants = append(variants, TileVariant{}) } variants[int(tv.Variant)] = tv seq[tv.Tag] = variants } for _, cg := range ent.CompactGenomes { if !matchGenome.MatchString(cg.Name) { continue } // pad to full slice size // to avoid out-of-bounds // checks later if sliceSize := 2 * int(cg.EndTag-cg.StartTag); len(cg.Variants) < sliceSize { cg.Variants = append(cg.Variants, make([]tileVariantID, sliceSize-len(cg.Variants))...) } cgs[cg.Name] = cg } return nil }) if err != nil { return err } tagstart := cgs[cmd.cgnames[0]].StartTag tagend := cgs[cmd.cgnames[0]].EndTag // TODO: filters log.Infof("%04d: renumber/dedup variants for tags %d-%d", infileIdx, tagstart, tagend) variantRemap := make([][]tileVariantID, tagend-tagstart) throttleCPU := throttle{Max: runtime.GOMAXPROCS(0)} for tag, variants := range seq { tag, variants := tag, variants throttleCPU.Go(func() error { count := make(map[[blake2b.Size256]byte]int, len(variants)) rt := reftile[tag] var rthash [blake2b.Size256]byte if rt != nil { rthash = blake2b.Sum256(rt.tiledata) count[rthash] = 0 } for _, cg := range cgs { idx := int(tag-tagstart) * 2 for allele := 0; allele < 2; allele++ { v := cg.Variants[idx+allele] if v > 0 && len(variants[v].Sequence) > 0 { count[variants[v].Blake2b]++ } } } // hash[i] will be the hash of // the variant(s) that should // be at rank i (0-based). hash := make([][blake2b.Size256]byte, 0, len(count)) for b := range count { hash = append(hash, b) } sort.Slice(hash, func(i, j int) bool { bi, bj := &hash[i], &hash[j] if ci, cj := count[*bi], count[*bj]; ci != cj { return ci > cj } else { return bytes.Compare((*bi)[:], (*bj)[:]) < 0 } }) // rank[b] will be the 1-based // new variant number for // variants whose hash is b. rank := make(map[[blake2b.Size256]byte]tileVariantID, len(hash)) for i, h := range hash { rank[h] = tileVariantID(i + 1) } // remap[v] will be the new // variant number for original // variant number v. remap := make([]tileVariantID, len(variants)) for i, tv := range variants { remap[i] = rank[tv.Blake2b] } variantRemap[tag-tagstart] = remap if rt != nil { rt.variant = rank[rthash] } return nil }) } throttleCPU.Wait() for tag := tagstart; tag < tagend; tag++ { rt, ok := reftile[tag] if !ok { // Excluded by specified // regions, or reference does // not use any variant of this // tile. (TODO: log this? // mention it in annotations?) continue } remap := variantRemap[tag-tagstart] maxv := tileVariantID(0) for _, v := range remap { if maxv < v { maxv = v } } variants := seq[tag] mtx.Lock() fmt.Fprintf(dumpVariantsW, "%d,%d,1,%s,%d,%s\n", tag, rt.variant, rt.seqname, rt.pos+1, bytes.ToUpper(rt.tiledata)) mtx.Unlock() done := make([]bool, maxv+1) for v, tv := range variants { v := remap[v] if v == 0 || v == rt.variant || done[v] { continue } else { done[v] = true } mtx.Lock() fmt.Fprintf(dumpVariantsW, "%d,%d,0,%s,%d,%s\n", tag, v, rt.seqname, rt.pos+1, bytes.ToUpper(tv.Sequence)) mtx.Unlock() } } log.Infof("%s: done (%d/%d)", infile, int(atomic.AddInt64(&done, 1)), len(infiles)) return nil }) } if err = throttleMem.Wait(); err != nil { return err } err = dumpVariantsW.Flush() if err != nil { return err } err = dumpVariantsF.Close() if err != nil { return err } return nil }