X-Git-Url: https://git.arvados.org/arvados.git/blobdiff_plain/783aff1187eba225008cd7db6fdd8307e46bc59b..d56b21609ddd2e2e096f5c30e991d24aa213f7f4:/crunch_scripts/split-fastq.py diff --git a/crunch_scripts/split-fastq.py b/crunch_scripts/split-fastq.py index f42b2b0ab9..61c384fbf0 100755 --- a/crunch_scripts/split-fastq.py +++ b/crunch_scripts/split-fastq.py @@ -1,107 +1,70 @@ #!/usr/bin/python +# Copyright (C) The Arvados Authors. All rights reserved. +# +# SPDX-License-Identifier: Apache-2.0 import arvados import re import hashlib +import string -#api = arvados.api('v1') +api = arvados.api('v1') piece = 0 manifest_text = "" -def readline(reader, start): - line = "" +# Look for paired reads + +inp = arvados.CollectionReader(arvados.getjobparam('reads')) + +manifest_list = [] + +def nextline(reader, start): n = -1 - while n == -1: - r = reader.readfrom(start, 1024) + while True: + r = reader.readfrom(start, 128) if r == '': break n = string.find(r, "\n") - line += r[0:n] - start += len(r) - return line - -def splitfastq(p): - for i in xrange(0, len(p)): - p[i]["start"] = 0 - p[i]["end"] = 0 - - while True: - recordsize = [0, 0] - - # read 4 lines starting at "start" - for ln in xrange(0, 4): - for i in xrange(0, len(p)): - r = readline(p[i]["reader"], p[i]["start"]) - if r == '': - return - recordsize[i] += len(r) - - splitnow = False - for i in xrange(0, len(p)): - if ((p[i]["end"] - p[i]["start"]) + recordsize[i]) >= arvados.BLOCKSIZE: - splitnow = True - - if splitnow: - for i in xrange(0, len(p)): - manifest = [] - manifest.extend(str(piece)) - manifest.extend([d[LOCATOR] for d in p["reader"]._stream._data_locators]) - manifest.extend(["{}:{}:{}".format(seg[LOCATOR], seg[BLOCKSIZE], self.name().replace(' ', '\\040')) for seg in arvados.locators_and_ranges(p[i]["reader"].segments, p[i]["start"], p[i]["end"] - p[i]["start"])]) - global manifest_text - manifest_text += manifest.join(" ") + "\n" - p[i]["start"] = p[i]["end"] + if n > -1: + break else: - for i in xrange(0, len(p)): - p[i]["end"] += recordsize[i] - -def put_manifest(manifest_text, sources=[]): - crm = arvados.CollectionReader(manifest_text) + start += 128 + return n - combined = crm.manifest_text(strip=True) - - m = hashlib.new('md5') - m.update(combined) - - uuid = "{}+{}".format(m.hexdigest(), len(combined)) - - collection = arvados.api().collections().create( - body={ - 'uuid': uuid, - 'manifest_text': crm.manifest_text(), - }).execute() - - for s in sources: - l = arvados.api().links().create(body={ - "link": { - "tail_uuid": s, - "head_uuid": uuid, - "link_class": "provenance", - "name": "provided" - }}).execute() - - return uuid - - -# Look for pairs - -#inp = arvados.CollectionReader(arvados.get_job_param('input')) - -with open("/home/peter/manifest") as f: - inp = arvados.CollectionReader(f.read()) - -prog = re.compile("(.*?)_1.fastq$") +prog = re.compile(r'(.*?)(_[12])?\.fastq(\.gz)?$') +# Look for fastq files for s in inp.all_streams(): - if s.name() == ".": - for f in s.all_files(): - result = prog.match(f.name()) - print f.name() - if result != None: - p = [{}, {}] - p[0]["reader"] = s.file(f) - p[1]["reader"] = s.file(prog.group(1) + "_2.fastq") - -print manifest_text - -#arvados.current_task().set_output(put_manifest(manifest_text)) + for f in s.all_files(): + name_pieces = prog.match(f.name()) + if name_pieces is not None: + if s.name() != ".": + # The downstream tool (run-command) only iterates over the top + # level of directories so if there are fastq files in + # directories in the input, the choice is either to forget + # there are directories (which might lead to name conflicts) or + # just fail. + print >>sys.stderr, "fastq must be at the root of the collection" + sys.exit(1) + + p = None + if name_pieces.group(2) is not None: + if name_pieces.group(2) == "_1": + p = [{}, {}] + p[0]["reader"] = s.files()[name_pieces.group(0)] + p[1]["reader"] = s.files()[name_pieces.group(1) + "_2.fastq" + (name_pieces.group(3) if name_pieces.group(3) else '')] + else: + p = [{}] + p[0]["reader"] = s.files()[name_pieces.group(0)] + + if p is not None: + for i in xrange(0, len(p)): + m = p[i]["reader"].as_manifest().split() + m[0] = "./_" + str(piece) + manifest_list.append(m) + piece += 1 + +manifest_text = "\n".join(" ".join(m) for m in manifest_list) + "\n" + +arvados.current_task().set_output(manifest_text)