#!/usr/bin/python
+# Copyright (C) The Arvados Authors. All rights reserved.
+#
+# SPDX-License-Identifier: Apache-2.0
import arvados
import subprocess
-import subst
+import crunchutil.subst as subst
import shutil
import os
+import sys
+import time
if len(arvados.current_task()['parameters']) > 0:
p = arvados.current_task()['parameters']
os.unlink("/usr/local/share/bcbio-nextgen/galaxy")
os.mkdir("/usr/local/share/bcbio-nextgen/galaxy")
-shutil.copy("/usr/local/share/bcbio-nextgen/galaxy/config/bcbio_system.yaml", "/usr/local/share/bcbio-nextgen/galaxy")
+shutil.copy("/usr/local/share/bcbio-nextgen/config/bcbio_system.yaml", "/usr/local/share/bcbio-nextgen/galaxy")
-os.mkdir("/usr/local/share/bcbio-nextgen/galaxy/tool-data")
-
-with open("/usr/local/share/bcbio-nextgen/galaxy/tool-data/tool_data_table_conf.xml") as f:
+with open("/usr/local/share/bcbio-nextgen/galaxy/tool_data_table_conf.xml", "w") as f:
f.write('''<tables>
<!-- Locations of indexes in the BWA mapper format -->
<table name="bwa_indexes" comment_char="#">
</tables>
''')
+os.mkdir("/usr/local/share/bcbio-nextgen/galaxy/tool-data")
+
with open("/usr/local/share/bcbio-nextgen/galaxy/tool-data/bowtie2_indices.loc", "w") as f:
- f.write(subst.do_substitution(p, "GRCh37\tGRCh37\tHuman (GRCh37)\t$(dir $(bowtie2_indices))"))
+ f.write(subst.do_substitution(p, "GRCh37\tGRCh37\tHuman (GRCh37)\t$(dir $(bowtie2_indices))\n"))
-with open("/usr/local/share/bcbio-nextgen/galaxy/tool-data/bwa_indices.loc", "w") as f:
- f.write(subst.do_substitution(p, "GRCh37\tGRCh37\tHuman (GRCh37)\t$(file $(bwa_indices))"))
+with open("/usr/local/share/bcbio-nextgen/galaxy/tool-data/bwa_index.loc", "w") as f:
+ f.write(subst.do_substitution(p, "GRCh37\tGRCh37\tHuman (GRCh37)\t$(file $(bwa_index))\n"))
with open("/usr/local/share/bcbio-nextgen/galaxy/tool-data/gatk_sorted_picard_index.loc", "w") as f:
- f.write(subst.do_substitution(p, "GRCh37\tGRCh37\tHuman (GRCh37)\t$(file $(gatk_sorted_picard_index))"))
+ f.write(subst.do_substitution(p, "GRCh37\tGRCh37\tHuman (GRCh37)\t$(file $(gatk_sorted_picard_index))\n"))
with open("/usr/local/share/bcbio-nextgen/galaxy/tool-data/picard_index.loc", "w") as f:
- f.write(subst.do_substitution(p, "GRCh37\tGRCh37\tHuman (GRCh37)\t$(file $(picard_index))"))
+ f.write(subst.do_substitution(p, "GRCh37\tGRCh37\tHuman (GRCh37)\t$(file $(picard_index))\n"))
with open("/usr/local/share/bcbio-nextgen/galaxy/tool-data/sam_fa_indices.loc", "w") as f:
- f.write(subst.do_substitution(p, "index\tGRCh37\t$(file $(sam_fa_indices))"))
+ f.write(subst.do_substitution(p, "index\tGRCh37\t$(file $(sam_fa_indices))\n"))
+
+with open("/tmp/crunch-job/freebayes-variant.yaml", "w") as f:
+ f.write('''
+# Template for whole genome Illumina variant calling with FreeBayes
+# This is a GATK-free pipeline without post-alignment BAM pre-processing
+# (recalibration and realignment)
+---
+details:
+ - analysis: variant2
+ genome_build: GRCh37
+ # to do multi-sample variant calling, assign samples the same metadata / batch
+ # metadata:
+ # batch: your-arbitrary-batch-name
+ algorithm:
+ aligner: bwa
+ mark_duplicates: true
+ recalibrate: false
+ realign: false
+ variantcaller: freebayes
+ platform: illumina
+ quality_format: Standard
+ # for targetted projects, set the region
+ # variant_regions: /path/to/your.bed
+''')
+
+os.unlink("/usr/local/share/bcbio-nextgen/gemini_data")
+os.symlink(arvados.get_job_param_mount("gemini_data"), "/usr/local/share/bcbio-nextgen/gemini_data")
os.chdir(arvados.current_task().tmpdir)
-rcode = subprocess.call(["bcbio_nextgen.py", "--workflow", "template", "gatk-variant", "project1",
- subst.do_substitution(p, "$(R1)"),
- subst.do_substitution(p, "$(R2)")])
+rcode = subprocess.call(["bcbio_nextgen.py", "--workflow", "template", "/tmp/crunch-job/freebayes-variant.yaml", "project1",
+ subst.do_substitution(p, "$(file $(R1))"),
+ subst.do_substitution(p, "$(file $(R2))")])
os.chdir("project1/work")
-rcode = subprocess.call(["bcbio_nextgen.py", "../config/project1.yaml"])
+os.symlink("/usr/local/share/bcbio-nextgen/galaxy/tool-data", "tool-data")
+
+rcode = subprocess.call(["bcbio_nextgen.py", "../config/project1.yaml", "-n", os.environ['CRUNCH_NODE_SLOTS']])
+
+print("run-command: completed with exit code %i (%s)" % (rcode, "success" if rcode == 0 else "failed"))
+
+if rcode == 0:
+ os.chdir("../final")
+
+ print("arvados-bcbio-nextgen: the follow output files will be saved to keep:")
+
+ subprocess.call(["find", ".", "-type", "f", "-printf", "arvados-bcbio-nextgen: %12.12s %h/%f\\n"])
+
+ print("arvados-bcbio-nextgen: start writing output to keep")
+
+ done = False
+ api = arvados.api('v1')
+ while not done:
+ try:
+ out = arvados.CollectionWriter()
+ out.write_directory_tree(".", max_manifest_depth=0)
+ outuuid = out.finish()
+ api.job_tasks().update(uuid=arvados.current_task()['uuid'],
+ body={
+ 'output':outuuid,
+ 'success': (rcode == 0),
+ 'progress':1.0
+ }).execute()
+ done = True
+ except Exception as e:
+ print("arvados-bcbio-nextgen: caught exception: {}".format(e))
+ time.sleep(5)
+
+sys.exit(rcode)
projects / arvados.git / blobdiff