X-Git-Url: https://git.arvados.org/arvados-tutorial.git/blobdiff_plain/5b4f2a85bedfad9e72e406e3e1990afc913c9da2..bec60d8f8671fedf159d9be0c02196ebaf602d53:/RNA-Seq/cwl/helper/alignment.cwl diff --git a/RNA-Seq/cwl/helper/alignment.cwl b/RNA-Seq/cwl/helper/alignment.cwl index 6712aae..e2a434f 100644 --- a/RNA-Seq/cwl/helper/alignment.cwl +++ b/RNA-Seq/cwl/helper/alignment.cwl @@ -12,7 +12,7 @@ requirements: steps: fastqc: - run: bio-cwl-tools/fastqc/fastqc_2.cwl + run: fastqc_2.cwl in: reads_file: fq out: [html_file] @@ -21,7 +21,7 @@ steps: requirements: ResourceRequirement: ramMin: 9000 - run: bio-cwl-tools/STAR/STAR-Align.cwl + run: STAR-Align.cwl in: RunThreadN: {default: 4} GenomeDir: genome @@ -29,12 +29,11 @@ steps: OutSAMtype: {default: BAM} SortedByCoordinate: {default: true} OutSAMunmapped: {default: Within} - ### 1. Expressions on step inputs OutFileNamePrefix: {valueFrom: "$(inputs.ForwardReads.nameroot)."} out: [alignment] samtools: - run: bio-cwl-tools/samtools/samtools_index.cwl + run: samtools_index.cwl in: bam_sorted: STAR/alignment out: [bam_sorted_indexed]