$namespaces: s: https://schema.org/ edam: http://edamontology.org/ arv: "http://arvados.org/cwl#" s:codeRepository: https://github.com/arvados/arvados-tutorial s:license: https://www.gnu.org/licenses/agpl-3.0.en.html cwlVersion: v1.1 class: CommandLineTool label: Convert one chromosome of bam to fastqs requirements: ShellCommandRequirement: {} hints: DockerRequirement: dockerPull: curii/bwa-samtools ResourceRequirement: ramMin: 20000 coresMin: 4 arv:RuntimeConstraints: keep_cache: 9216 outputDirType: keep_output_dir SoftwareRequirement: packages: Samtools: specs: [ "https://identifiers.org/rrid/RRID:SCR_002105" ] version: [ "1.10" ] inputs: bam: type: File #format: edam:format_2572 # BAM label: Alignments in BAM format secondaryFiles: [.bai] sample: type: string label: Sample name chrom: type: string label: Chromosome name outputs: fastq1: type: File #format: edam:format_1930 # FASTQ label: One of set of pair-end FASTQs (R1) outputBinding: glob: "*_1.fastq.gz" fastq2: type: File #format: edam:format_1930 # FASTQ label: One of set of pair-end FASTQs (R2) outputBinding: glob: "*_2.fastq.gz" baseCommand: [samtools, view] arguments: - "-b" - $(inputs.bam) - $(inputs.chrom) - shellQuote: false valueFrom: "|" - "samtools" - "sort" - "-n" - "-" - shellQuote: false valueFrom: "|" - "samtools" - "fastq" - "-@" - $(runtime.cores) - "-" - "-N" - prefix: "-0" valueFrom: "/dev/null" - prefix: "-s" valueFrom: "/dev/null" - prefix: "-1" valueFrom: $(inputs.sample)_1.fastq.gz - prefix: "-2" valueFrom: $(inputs.sample)_2.fastq.gz